| Trade - Want to Buy |
| Topic | Urea Assay Kit |
| Name | NICOE |
| Company | REAGEN LLC |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | usa |
| Date Created | 9/30/2013 9:23:56 AM |
| Message | Product Description
REAGEN™ Urea Assay Kit is a cuvette-based enzymatic assay for the determination of urea across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of urea directly from food samples.
The unique features of the kit are:
High sensitivity and low detection limit (0.6 ppm)
High reproducibility
A rapid (30 minute), robust enzyme-based assay
Procedure Overview
REAGEN™ Urea Assay Kit uses a coupled enzymatic reaction scheme to detect urea in food samples. The assay measures the concentration of urea using the urease enzyme which converts urea to ammonia. The ammonia produced by this reaction is then used as a substrate by the enzyme glutamate dehydrogenase to make glutamate. In the process, NADH is stoichiometrically converted to NAD+. This conversion, measured at 340 nm, is proportional to the level of urea in the sample.
The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of urea in each sample is then directly determined from the change in NADH absorbance at 340 nm within 25 minutes.
Kit Contents, Storage and Shelf Life
REAGEN™ Urea Assay Kit has the capacity for 48 determinations or 24 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 12 months, after receipt, when the kit is properly stored.
Kit Contents Amount Storage
Substrate Solution One vial 4ºC
Urease 0.25 mL 4ºC
Microtiter plate One Room temp or 4ºC
Sensitivity (Detection Limit)
Sample Volume Detection Limit (mg/L)
0.01 mL 12
0.2 mL 0.6
The linear range of the assay is 0.6 – 600 mg/L (ppm).
Required Materials Not Provided With the Kit
Spectrophotometer (340 nm)
Low volume (0.3 mL) UV cuvettes, such as Brand Scientific catalog #7592 00
Deionized or distilled water
50 mL plastic conical tubes
Warnings and Precautions
Do not use the kit past the expiration date.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulate material under the assay plates during incubation.
Make sure you are using only distilled or deionized water since water quality is very important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
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