| Trade - Want to Buy |
| Topic | Fluoroquinolone Strip Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | USA |
| Date Created | 9/28/2013 2:01:17 PM |
| Message | Product description
REAGEN™ Fluoroquinolone Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect fluoroquinolone antibiotic residues in raw commingled cow’s milk. This state-of-the-art test uses a broad-spectrum antibody capable of specifically detecting a range of fluoroquinolone residues. This test is designed for point-of-care use or reference lab settings and eliminates the need for a heating block.
The features of the kit are:
Rapid strip test method – 7 minutes
Broad-spectrum antibody that requires no heating step
High sensitivity
High reproducibility
Mechanism of reaction
The assay uses a competitive colloidal gold based format.The milksample(200μL)is added to a clear plastic reaction vessel,and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells.The milk is incubated briefly(3 min)to allow the fluoroquinolone antibody on the gold particles to engage with any fluoroquinolone antibiotic residues present in the milk.The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip.Any gold particles that are not complexed with antibiotics present in the milk will bind to the fluoroquinolone antibiotic imprinted at the Test line(T-line),forming a signal(red line)at that position.If the fluoroquinolone antibody on the gold particle has engaged with antibiotic present in the milk sample,the gold particle will flow past the T-line and reach the Control line(C-line).For visual interpretation of the test results,T-line signal intensity that is stronger than the signal at the C-line indicates a negative result.Signal at the T-line which is equal or less intense compared to the C-line indicates the presence of fluoroquinolone antibiotics in the milk.The greater the reduction in signal intensity at the T-line,the greater the concentration of fluoroquinolone antibiotic residues present in the milk.
Sensitivity (detection limit)
Fluoroquinolones
Antibiotic (MRL) Detection limit (μg/L)
Enrofloxacin (100) 1.5-2.5 ppb
Ciprofloxacin(100) 1-2 ppb
Difloxacin 20-30 ppb
Sarafloxacin 20-30 ppb
Norfloxacin 1-2 ppb
Marbofloxacin (75) 4-6 ppb
Danofloxacin (30) 2-3 ppb
Flumequine (50) 4-6 ppb
Kit Contents, Storage and Shelf Life
The REAGEN™ Fluoroquinolone Strip Test Kit has the capacity for 96 (RNS92009-01) or 192 (RNS92009-02) determinations. The shelf life is 24 months when the kit is properly stored.
The positive and negative control vials contain lyophilized raw milk samples, and must be reconstituted with 1 mL of distilled water before use. The reconstituted raw milk controls are stable for 48 hours at 4°C. Additional positive and \ negative control vials are available separately .
RNS92009-01
Kit Contents Amount Storage
Reaction wells 6 x 16 (96 total) 4°C
Test strips 6 x 16 strips (96 total)
Positive control (10 ppb Norfloxacin +/- 0.5 ) 1 vial
Negative control 1 vial
Pipette tips 100 tips RT
RNS92009-02
Kit Contents Amount Storage
Reaction wells 12 x 16 (192 total) 4°C
Test strips 12 x 16 strips (192 total)
Positive control (10 ppb Norfloxacin +/- 0.5 ) 2 vials
Negative control 2 vials
Pipette tips 200 tips RT
A 96-well plastic frame is also provided in each kit to anchor the reaction vessels in place during testing, and can be re-used as required.
Required Materials Not Provided With the Kit
Timer/Watch
Additional positive and negative controls
Polypropylene tubes, clean vials, or small glass test tubes
Distilled water
Scissors to cut strip caps
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