| Trade - Want to Buy |
| Topic | Sulfonamide Strip Test Kit |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | USA |
| Date Created | 9/28/2013 2:00:05 PM |
| Message | Product Description
REAGEN™ Sulfonamide Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect sulfonamide antibiotic residues in raw commingled cow’s milk. This rapid test uses a broad-spectrum binding protein which eliminates the need for a heating block, and is designed for rapid field.Examples of sulfonamide antibiotics and their limits of detection using the Sulfonamide Strip Test Kit are shown in Table 1.
The features of the kit are:
• Rapid strip test method –7 minutes
• Novel binding protein that requires no heating step
• High sensitivity
• High reproducibility
Mechanism of Reaction
The assay uses a competitive colloidal gold based format. The milk sample (200 μL) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The milk is incubated briefly (3 min) to allow the sulfonamide binding protein on the gold particles to engage with any sulfonamide antibiotic residues present in the milk. The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the milk will bind to the sulfonamide antibiotic imprinted at the Test line (T-line), forming a signal (red line) at that position. If the sulfonamide binding protein on the gold particle has engaged with antibiotic present in the milk sample, the gold particle will flow past the T-line and reach the Control line (C-line). For visual interpretation of the test results, T-line signal intensity that is stronger than the signal at the C-line indicates a negative result. Signal at the T-line which is equal or less intense compared to the C-line indicates the presence of sulfonamide antibiotic residues in the milk. The greater the reduction in signal intensity at the T-line, the greater the concentration of sulfonamide antibiotics present in the milk.
Sensitivity (Detection Limit)
Table 1:Detection limit by visual interpretation method for different sulfonamide antibiotics by Sulfonamide Strip Test Kit
Sulfonamides
Antibiotic (MRL) Detection limit (μg/L)
Sulfamethazine (100) 50
Sulfamethoxazole (100) 50
Sulfadiazine (100) 50
Sulfathiazole (100) 50
Sulfadimethoxine (100) 50
Sulfaquinoxaline (100) 50
Sulfapyridine (100) 50
Kit Contents, Storage and Shelf Life
REAGEN™Sulfonamide Strip Test Kit has the capacity for 96(RNS92003-01) or 192 (RNS92003-02) determinations. The shelf life is 24 months when the kit is properly stored.
The positive and negative control vials contain lyophilized raw milk samples, and must be reconstituted with 1 mL of distilled water before use. The reconstituted raw milk controls are stable for 48 hours at 4°C. Additional positive and negative control vials are available separately
RNS92003-01
Kit Contents Amount Storage
Reaction wells 6 x 16 (96 total) 4°C
Test strips 6 x 16 strips (96 total) 4°C
Positive control (100 ppb Sulfamethazine +/-5ppb) 1 vial 4°C
Negative control 1 vial 4°C
Pipette tips 100 tips RT
RNS92003-02
Kit Contents Amount Storage
Reaction wells 12 x 16 (192 total) 4°C
Test strips 12 x 16 strips (192 total) 4°C
Positive control (100ppb Sulfamethazine +/-5ppb) 2 vials 4°C
Negative control 2 vials 4°C
Pipette tips 200 tips RT
A 96-well plastic frame is also provided in each kit to anchor the reaction vessels in place during testing, and can be re-used as required.
Required Materials Not Provided With the Kit
Timer/Watch
Additional positive and negative controls
Polypropylene tubes, clean vials, or small glass test tubes
Distilled water
Scissors to cut strip wells
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