| Message | Product Description
REAGEN™ Melamine Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect melamine residues in raw commingled cow’s milk and processed milk products. This one-step test requires no sample preparation and is designed for point-of-care use or reference lab settings
The unique features of the kit are:
• Rapid strip test method – 7 minutes
• No sample preparation required
• High sensitivity
• High reproducibility
Mechanism of Reaction
The assay uses a competitive colloidal gold based format. The milk sample (200 μL) is added to a clear plastic strip well or tube and the test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with melamine present in the milk will bind to the melamine imprinted at the Test line (T-line), forming a signal (red line) at that position. If the antibody on the gold particle has engaged with melamine present in the milk sample, the gold particle will flow past the T-line and reach the Control line (C-line). For visual interpretation of the test results, T-line signal intensity that is stronger than the signal at the C-line indicates a negative result. Signal at the T-line which is equal or less intense compared to the C-line indicates the presence of melamine in the milk. The greater the reduction in signal intensity at the T-line, the greater the concentration of melamine present in the milk.
sensitivity (Detection Limit)
The sensitivity of the kit in raw commingled cow’s milk is 100 ppb.
Kit Contents, Storage and Shelf Life
The REAGEN™ Melamine Strip Test Kit has the capacity for 96(RNS92006-01) or 192 (RNS92006-02) determinations. The shelf life is 12 months when the kit is properly stored.
The positive and negative control vials contain lyophilized raw milk samples, and must be reconstituted with 1 mL of distilled water before use. The reconstituted raw milk controls are stable for 48 hours at 4°C. Additional positive and negative control vials are available separately
RNS92006-01
Kit Contents Amount Storage
Test strips with empty strip wells 6 x 16 strips (96 total) 4°C
Positive control (200 ppb melamine +/- 5 ppb) 1 vial
Negative control 1 vial
Pipette tips 100 tips RT
RNS92006-02
Kit Contents Amount Storage
Test strips with empty strip wells 12 x 16 strips (192 total) 4°C
Positive control (200 ppb melamine +/- 5 ppb) 2 vials
Negative control 2 vials
Pipette tips 200 tips RT
A 96-well plastic frame is also provided in each kit to anchor the reaction vessels in place during testing, and can be re-used as required.
Required Materials Not Provided With the Kit
Timer/Watch
Additional positive and negative controls (Cat # 109110)
Polypropylene tubes, clean vials, or small glass test tubes
Distilled water
Scissors to cut strip wells
ELISA Testing Protocol
1. Using scissors, carefully cut the number of empty strip wells and remove test strips appropriate for the number of samples that will be tested and allow reagents to reach ambient temperature (25˚C +/- 5). Place the empty strip wells into the 96-well plastic frame to anchor the wells in place during testing. Promptly re-store the remaining components at 4˚C.
2. Shake the milk vigorously, aliquot sample into a small polypropylene tube or clean vial (1.5 mL – 15 mL), and allow milk sample to reach ambient temperature (25˚C +/- 5). For faster milk temperature equilibration, pipette 1 mL of cold milk into a small glass test tube. The milk should reach the minimal 20˚C in less than 5 min.
3. Insert the pipet tip into the milk sample, depress the plunger of the pipettor to the first stop, then slowly release the plunger to aspirate 200 μL of milk into the pipet tip.
4. Insert the bottom of the test strip into the well containing the milk sample (see Figure 1). The strip should be inserted such that the arrows are pointing down. Set a timer for 7 min.
5. After 7 min, remove the strip and place it on a horizontal surface with the unmarked side facing up.
6. Visually examine the intensity of the signal at the T-line and C-line.
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