| Trade - Want to Buy |
| Topic | BT Combo Strip Test |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/28/2013 1:37:43 PM |
| Message | Product Description
REAGEN™ BT Combo Strip Test is a qualitative and rapid lateral flow assay designed to detect both beta-lactam and tetracycline antibiotic residues in raw commingled cow’s milk. This test uses a tetracycline receptor and a novel beta-lactam binding protein which eliminates the need for a heating block. This test is designed for point-of-care use or reference lab settings.
The unique features of the kit are:
Rapid strip test method – 7 minutes
Novel binding protein that requires no heating step
High sensitivity
High reproducibility
Mechanism of Reaction
C-line indicates the presence of the corresponding antibiotic in the milk. The greater the reduction in signal intensity at the test line, the greater the concentration of tetracycline or beta-lactam antibiotics present in the milk. The assay is also designed to simultaneously detect tetracycline and
beta-lactam antibiotics.
Kit Contents, Storage and Shelf Life
The REAGEN™ BT Combo Strip Test has the capacity for 96(RNS92005-01) or 192 (RNS92005-02) determinations. The shelf life is 12 months when the kit is properly stored. The positive and negative control vials contain lyophilized raw milk samples, and must be reconstituted with 1 mL of distilled water before use. The positive control vial is spiked with 4 ppb Penicillin G and 100 ppb of Oxytetracycline. The reconstituted raw milk controls are stable for 48 hours at 4°C. Additional positive and negative control vials are available separately
RNS92005-01
The assay uses a competitive colloidal gold based format. The milk sample (200 μL) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The milk is incubated briefly (3 min) to allow the both antibiotic binding proteins on the gold particles to engage with any antibiotic present in the milk.
The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the milk will bind to their respective test line, forming a signal (red line) at that position. Each test strip is imprinted with two test lines and one Control line (C -line). One test line allows for the detection of tetracyclines, and the other for the detection of beta -lactam antibiotics. If the binding protein on the gold particle has engaged with either a tetracycline or a beta-lactam antibiotic present in the milk sample, the gold particle will flow past the test line corresponding to that antibiotic and reach the C-line. For visual interpretation of the test results, any test line signal intensity that is stronger than the signal at the C -line indicates a negative
result. Signal at any test line which is equal or less intense compared to th e
RNS92005-02
Kit Contents Amount Storage
Reaction wells 12 x 16 (192 total)
4°C
Test strips 12x 16 strips(192 total)
Positive control 2 vials
Negative control 2 vials
Pipette tips 200 tips RT
A 96-well plastic frame is also provided in each kit to anchor the reaction vessels in place during testing, and can be re-used as required.
Sensitivity (Detection Limit)
Beta-lactam Antibiotics
Tetracycline Antibiotics
Penicillins Cefalosporins
Antibiotic
(MRL) Detectionli mit( ppb) Antibiotic
(MRL) Detection limit (ppb) Antibiotic (MRL) Detection limit ppb)
PenicillinG (4)
3-4 Ceftiofur
(100)
75-100 Tetracycline
(100)
50-75
Ampicillin
(4)
4-5 Cephapirin
(60)
6-15 Chlorotetracycline
(100)
20-50
Amoxicillin
(4)
4-5 Cefazolin
(50)
35-50 Doxycycline
(100)
10-35
Cloxacillin
(30)
4-8 Cefoperazone
(50)
5-20 Oxytetracycline
(100)
30-55
Oxacillin
(30)
4-8 Cephquinome
(20) 10-20
Dicloxacillin
(30)
5-8 Cephalonium
(10)
3-10
Nafcillin
(30)
15-30
Required Materials Not Provided With the Kit
Timer
Additional positive and negative controls
Polypropylene tubes
Distilled water
Scissors to cut strip caps
Fixed-volumer pipettor to deliver 200 μL
Optional Materials Not Provided With the Kit
Detekt RDS-1000 test strip reader
Fixed-volumer pipettor to deliver 200 μL
Note: this product was developed using the Detekt RDS-1000 reader, and is highly recommended for optimal performance. For customers already possessing the Detekt RDS-1000 reader, or wishing to use an alternate reader, please contact REAGEN at [email protected] for instructions on how to program the reader for use
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