| Trade - Want to Buy |
| Topic | Salmonella ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | USA |
| Date Created | 9/28/2013 1:21:48 PM |
| Message | Product Description
REAGEN™Salmonella ELISA Test Kit is designed for routine screening for Salmonella from cultured food and feed samples. Salmonella is a genus of gram negative bacteria belonging to the Enterobacteriaceae family. As a major contributor to foodborne illness, Salmonella is founded in poultry, meat and eggs. When Salmonella is consumed it may result in the disease known as Salmonellosis. The detection of Salmonella in food samples is often laborious and time-consuming. Several days are required to culture the bacteria from a sample followed by a final isolation and identification by biochemical and serological techniques. The REAGEN™Salmonella ELISA Test Kit provides a simple, rapid, sensitive and cost-effective enzyme immunoassay (ELISA) screening method, which enables government agencies,seafood processors,as well as quality assurance organizations,to detect positive and negative Salmonella samples to as low as 105 cells/mL level.
Procedure Overview
The method is based on a colorimetric ELISA assay. The monoclonal antibody against Salmonella has been coated in the plate wells. During the analysis, the sample is added. If Salmonella is present in the sample, it will bind with the antibody.A suitable broth is added to the well for rapid growth.The secondary antibody, tagged with a peroxidase enzyme, targets the Salmonella that is complexed to the primary antibody coated on the plate wells. The resulting color intensity, after addition of substrate, is related to the amount of Salmonella in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Salmonella ELISA Test Kit has the capacity for 96 determinations or testing of 46 samples in duplicate (assuming 4 wells for positive and negative controls). Most of the kit components should be stored at 2-8 C except for the Salmonella controls and 50X Ab#2 stock which should be stored at -20 C. Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Salmonella antibody-coated Plate 1x 96-well Plate(8 wells x 12 strips) 2-8C
1X Salmonella Negative Control 1.0 mL -20C
1X Salmonella Positive Control 1.0 mL -20C *
HRP-Conjugated Antibody #2 (powder) 3 tubes -20C
Antibody #2 Diluent
20X Wash Solution
Stop Buffer
TMB Substrate 20 mL
30 mL
20 mL
10 mL 2-8C
Sensitivity
The kit can detect 105cells/mL
Specificity
The kit can broadly detect Salmonella serotypes of group A, B, C, D, E and other Salmonella antigen classes. The intensity of reaction may vary with the strain of Salmonella tested
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Modified GN Broth: GN broth containing 10µg/ml Novobiocin. GN broth can be purchased or prepare in-house per the following protocol.
Gram Negative Broth (GN Broth or Hajna GN Broth, pH 7.0 ± 0.2)：
Tryptose . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . .. . . . . 20.0g
Dex. . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.0g D-Mannitol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . 2.0g Sodiumcitrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.0 g Sodium desoxycholate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.5 g Dipotassium phosphate . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.0 g Monopotassium phosphate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.5 g Sodium chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.0 g Distilled water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.0 L
（Dissolve ingredients in water. Autoclave at 121°C for 15 min. Avoid overheating）
Autoclave or boiling water bath (100°C)
Culture bottles (500ml)
Microbiological media for enrichment of Salmonella
Microbiological media for the confirmation of Salmonella
Distilled water
Warnings and Precautions
The samples contain Salmonella. Handle with particular care.
Good microbiological practices (i.e. aseptic technique, safety precautions) should be employed when carrying out sample preparation and enrichment procedures.
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or lots except for components with the same part No’s within their expiration dates.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulation material under the assay plates during incubation.
Make sure you are using only distilled or deionized water since water quality is very important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards first and then your samples.
Add standards to plate only in the order from low concentration to high concentration as this will minimize the risk of compromising the standard curve.
Always refrigerate plates in sealed bags with a desiccant to maintain stability. Prevent condensation from forming on plates by allowing them equilibrate to room temperature (20 – 25C / 68 – 77F) while in the packaging.
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