| Trade - Want to Buy |
| Topic | Beta-Agonist ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/28/2013 1:08:32 PM |
| Message | Product Description
REAGEN™ Beta-Agonist ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Beta-Agonist and other related ß-agonists in feed, meat/liver/kidney, milk and urine.Beta-Agonist ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect Beta-Agonist in various sample types in response to customer concerns about food safety.
The unique features of the kit are:
Rapid (10 - 30 minutes), and organic reagent-free extraction method for various samples with high recovery (75 - 105%).
High sensitivity (0.05 ng/g or ppb) and low detection limit (0.025 ng/g or ppb for meat/tissue, 0.05 ng/g or ppb for urine and 0.2 ng/g or ppb for feed).
High reproducibility.
A quick ELISA assay (less than 1.5 hours regardless of number of samples.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
Kit Contents Amount Storage
Beta-Agonist coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
Beta-Agonist Standards:
Negative control (white cap tube)
0.05 ng/mL (yellow cap tube)
0.15 ng/mL (orange cap tube)
0.5 ng/mL (pink cap tube)
1.5 ng/mL (purple cap tube)
4.5 ng/mL (blue cap tube)
10 ng/mL (spiking, optional, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
2-8C
Beta-Agonist Antibody #1 15 mL 2-8C
100X HRP-Conjugated Antibody #2 250 L
Antibody #2 Diluent ** 20 mL
20X Wash Solution ** 30 mL
Stop Buffer ** 20 mL
TMB Substrate ** 10 mL
Sample Balance Buffer **
10X PBS **
200X Sample Extraction Buffer ** 0.5 mL
25 mL
25 mL
* If you are not planning to use the kit for over 1 month, store Beta-Agonist Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type feed Meat/Liver/Kidney milk urine
Clenbuterol 0.2 0.025 0.25 0.05
Bitolterol 0.2 0.025 0.25 0.05
Carbuterol 0.2 0.025 0.25 0.05
Salbutamol 0.2 0.025 0.25 0.05
Cimbuterol 0.2 0.025 0.25 0.05
Mabuterol 0.2 0.025 0.25 0.05
Terbutaline 0.2 0.025 0.25 0.05
Bromobuterol 0.2 0.025 0.25 0.05
Timolol 10.6 1.25 12.6 3.0
Specificity
Analytes Cross-Reactivity (%)
Clenbuterol 100
Bitolterol 100
Carbuterol 100
Salbutamol 100
Cimbuterol 100
Mabuterol 100
Terbutaline 100
Bromobuterol 100
Timolol 10
Metaproterenol 1.0
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
normal hexane
ethyl acetate
acetonitrile
Warnings and Precautions
The standards contain Beta-Agonist. Handle with particular care.
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or lots except for components with the same part No’s within their expiration dates. ANTIBODIES AND PLATES ARE KIT-AND LOT-SPECIFIC. Make sure that the antibody #2 and diluent are mixed in correct volumes.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulation material under the assay plates during incubation.
Make sure you are using only distilled or deionized water since water quality is very important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards first and then your samples.
Add standards to plate only in the order from low concentration to high concentration as this will minimize the risk of compromising the standard curve.
Always refrigerate plates in sealed bags with a desiccant to maintain stability. Prevent condensation from forming on plates by allowing them equilibrate to room temperature (20 – 25C / 68 – 77F) while in the packaging.
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