| Trade - Want to Buy |
| Topic | Saxitoxin (PSP) ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/28/2013 12:59:36 PM |
| Message | Product Description
REAGEN™ Saxitoxin (PSP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Saxitoxin in mussels and water samples. Saxitoxin, the principal component of paralytic shellfish poisons (PSP), blocks the sodium ion channels in nerve and muscle membranes.The lethal dose in humans is 1 to 3 mg. Numbness and respiratory paralysis can occur after oral ingestion of as little as 0.5 - 1.0g saxitoxin.
Saxitoxin residues can be determined by either GC-MS or HPLC. However, enzyme immunoassays (ELISA) show considerable advantages as it is faster and more sensitive.
REAGEN™ Saxitoxin (PSP) ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect saxitoxin in shellfish matrices and to satisfy customer concerns about food safety.
The unique features of the kit are:
High recovery (80%-105%), rapid (10 - 30 minutes), and cost-effective extraction methods
High sensitivity (0.05 ng/g or ppb) and low detection limit (3 ppb for mussel samples and 1 ppb for water samples).
High reproducibility.
A quick ELISA assay(less than 1.5 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The second antibody against anti-saxitoxin antibody was coated in the plate wells. During the analysis, sample is added along with the saxitoxin-horseradish peroxidase (Saxitoxin-HRP) conjugate and anti-saxitoxin antibody. If the saxitoxin residue is present in the sample, it will compete with Saxitoxin-HRP for the anti-saxitoxin antibody, thereby preventing the Saxitoxin-HRP from binding to the anti-Saxitoxin antibody. The anti-saxitoxin antibodies are then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the saxitoxin residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Saxitoxin (PSP) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Second antibody-Coated Plate 1 x 96-well Plate(8 wells x 12 strips) 2-8C
Saxitoxin (PSP) Standards:
Negative control (white cap tube)
0.05 ng/mL (yellow cap tube)
0.15 ng/mL (orange cap tube)
0.5 ng/mL (pink cap tube)
1.5ng/mL (purple cap tube)
4.5ng/mL (blue cap tube)
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8mL
0.8 mL -20C
Anti-Saxitoxin Antibody 6 ml 2-8C
Saxitoxin-HRP Conjugated 6 mL
20X Wash Solution 28 mL
Stop Buffer 14mL
TMB Substrate 12 mL
10X Sample Extraction Buffer 25 mL
If you are not planning to use the kit for over 1 months, store Anti-Saxitoxin Antibody and HRP-Conjugated at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Mussels 3
Water 1
Specificity
Analytes Cross-Reactivity (%)
Saxitoxin 100
Neosaxitoxin 20
Decarbamoyl Saxitoxin 100
GTX 2 & 3 43
GTX 1 & 4 2
Decarbamoyl GTX 2 & 3 10
Decarbamoyl Neosaxitoxin 4
GTX-5 61
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer
Vortex mixer
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Methanol
Warnings and Precautions
The standards contain a small amount of Saxitoxin . Handle with particular care.
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or lots except for components with the same part No’s within their expiration dates.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulation material under the assay plates during incubation.
Make sure you are using distilled or deionized water since water quality is important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards first and then your samples.
Add standards to plate only in the order from low concentration to high concentration as this will minimize the risk of compromising the standard curve.
Always refrigerate plates in sealed bags with a desiccant to maintain stability. Prevent condensation from forming on plates by allowing them equilibrate to room temperature (20 – 25C / 68 – 77F) while in the packaging.
|
| |
|
|
