| Trade - Want to Buy |
| Topic | Tetrodotoxin (TTX) ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | USA |
| Date Created | 9/28/2013 12:54:49 PM |
| Message | GENERAL DESCRIPTION
Tetrodotoxin (TTX) is a powerful neurotoxin, which is tolerance to heat, salt and cooking .Minimum lethal dose for human is about 0.5mg/60 kg of body weight, The toxicity is 1000 times great than the sodium cyanide. Every year many people are ill due to improper eating or eating puffer fish.Therefore, it is significance to accurate detection of tetrodotoxin in puffer fish in order to prevention and control of tetrodotoxin poisoning . The detection method is sensitive, fast, simple and specific, only need a small amount of fish sampling .
ASSAY PRINCIPLES
The REAGEN™Tetrodotoxin indirect competitive ELISA is an immunoassay for the quantitative and sensitive detection of Tetrodotoxin in water samples and puffer fish samples . Antigen is coated in test wells ,The sample extract and calibrators are pipetted into the test wells followed by TTX monoclone antibody into the test wells to initiate the reaction. After this 90 minutes incubation the wells are washed with wash solution.Then add enzymes labeled anti-mouse IgG antibody, incubation,then adding the color solution, terminate the reaction by the stop solutions, measured the amount of color in each well with a microplate reader at OD 450nm The color of the unknown samples is compared to the color of the calibrators and the TTX concentration of the samples is derived.
REAGENTS AND MATERIALS PROVIDED
The REAGEN™Tetrodotoxin ELISA kit in its original packaging can be used until the end of the month indicated on the box label whenstored at 2- 8ºC.
1. Plate containing 12 test strips of 8 wells each vacuum-packed in aluminized pouch with indicating dessicant.
2. 5 vials each containing 0.5 mL of TTX calibrators corresponding to 0, 10, 20, 50 and 200 ppb.
3. 1 vial containing 6mL of TTX antibody Solution.
4. 2 vial containing 6mL of enzyme conjugate Solution
5. 1 vial containing 12 mL of colour solution.
6. 1 vial containing 6 mL of Stop Solution. (Caution! 1N H2SO4. Handle with care.)
7. 1 vial containing 30 mL of Wash Solution concentrate 10X. Needs to be diluted prior to use.
8. Instructions
MATERIALS REQUIRED BUT NOT PROVIDED
1. Microplate Microplate Reader (including 450nm),
2. centrifuge
3. electric stove
4. micro pipette
5. magnetic stirrer
6. Graduated cylinder
7. funnel
8. flask
9. rapid qualitative filter paper
10.separating funnel
11. Acetic acid
12. sodium hydroxide
13. deionized water or distilled water
14. PBS buffer solution
PRECAUTIONS
1. The standard solutions of the test kit contain small amounts of TTX, Handle with care.
2. The Stop Solution is 0.5M sulphuric acid. Avoid contact with skin and mucous membranes.
3. Please put all the reagents back into the 2 ~ 8 ℃ after every Reagent addition steps.
4. Every reagent addition steps should be complete in 5 minutes.
5. Protect the strips from direct sunlight during Incubation.
6. Do not use reagents after expiration date.When OD450 of the standard 1 less than 0.5 ,zhe reagent may be degenerate.
7. Do not combine reagents from other different Lot numbers.
STORAGE AND STABILITY
The TTX ELISA Kit should to be stored in the refrigerator (2–8°C). Do not freeze.Substrate is sensitive to light，Protect the Substrate from direct sunlight.Remove the number of microtiter plate strips required from the aluminum pouch. The remaining strips are stored in the aluminum pouch and zip-locked closed. Store the remaining kit in the refrigerator (2-8°C).
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