| Trade - Want to Buy |
| Topic | Deoxynivalenol (DON) Column Kit |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | USA |
| Date Created | 9/28/2013 12:53:15 PM |
| Message | Product Description
REAGEN™ Deoxynivalenol (DON) Column Kit contains immunoaffinity columns for the convenient purification and enrichment of Deoxynivalenol (also known as Vomitoxin or DON) from a variety of food and feed samples for downstream HPLC and ELISA analysis. DON is a naturally-occurring fungal toxin that can be present in many different types of food. Contamination of food with DON can cause a variety of serious illnesses. The columns contain immobilized antibodies which bind DON. When food sample extracts are passed through the columns, DON will specifically bind to the column while the other substances pass through. The analyte is then eluted from the column using methanol.
Procedure Overview
REAGEN™ immunoaffinity columns use a simple procedure to enrich DON from food samples. A liquid extract prepared from the sample is passed through the column to bind the DON. After washing the column with a wash solution, the DON is eluted using methanol.The enriched samples are suitable for subsequent analysis by HPLC or ELISA methods. Each column is disposable and should only be used once.
Kit Contents, Storage and Shelf Life
REAGEN™ Deoxynivalenol (DON) Column Kit contains 25 columns. Store the kit at room temperature (18 - 25°C). The shelf life is 18 months when the kit is properly stored.
Kit Contents Amount Storage
columns 25 Room temp
Required Materials Not Provided With the Kit
50 mL plastic screwcap tubes
Deionized water
Acetonitrile
Methanol
Pipettes and pipette tips
Column stand or vacuum manifold
Homogenizer, food processor, or grain mill
Fluted filter paper (Whatman 40)
Glass microfiber filter
Nitrogen evaporator or vacuum centrifuge
Warnings and Precautions
Do not use the kit past the expiration date.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F).
Use only distilled or deionized water to prepare wash solutions; water quality is very important.
Do not allow the columns to be stored at temperatures above 30°C.
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