| Trade - Want to Buy |
| Topic | Aflatoxin M1 ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
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| Country | USA |
| Date Created | 9/28/2013 12:22:57 PM |
| Message | Product Description
REAGEN™ Aflatoxin M1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin M1 in cheese milk and milk powder.
The unique features of the kit are:
No need to extract the sample. The sample can be directly used for the ELISA
High sensitivity (0.005 ng/g or ppb) and low detection limit in milk(0..005ppb).
High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The Aflatoxin M1 antibody has been coated in the plate wells. During the analysis, sample is added to the wells for incubation. After washing the plate, the Aflatoxin M1 -horseradish peroxidase (Aflatoxin M1-HRP) conjugate is added to the wells for incubation. If the Aflatoxin M1 residue is present in the sample, it will compete for the Aflatoxin M1 antibody, thereby preventing the Aflatoxin M1-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Aflatoxin M1 residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Aflatoxin M1 ELISA Test Kit has the Aflatoxin M1acity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Aflatoxin M1 Antibody-Coated Plate 1 x 96-well Plate(8 wells x 12 strips) 2-8C
Aflatoxin M1 Standards:
Negative control (white cap tube)
0.005ng/mL (yellow cap tube)
0.015ng/mL (orange cap tube)
0.03 ng/mL (pink cap tube)
0.09ng/mL (purple cap tube)
0.27ng/mL (blue cap tube)
10 ng/mL (spiking, red cap tube)
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL
1.8 mL 2-8C
Aflatoxin M1-HRP Conjugated 12 mL 2-8C
20X Wash Solution ** 28 mL 2-8C
TMB Substrate 12 mL 2-8C
Stop Buffer ** 14mL 2-8C
10X PBS (Optional)** 25 mL 2-8C
10X Milk Extraction Buffer(Optional) 5 mL 2-8C
If you are not planning to use the kit for over 3 months, store Aflatoxin M1 standards and Aflatoxin M1-HRP Conjugated at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Milk 0.005
Milk powder 0.005
(corresponding to the dissolved liquid milk; 1:10)
Cheese 0.05
Yogurt/Butter milk/Soured milk 0.01
Specificity
Analytes Cross-Reactivity (%)
Aflatoxin M1 100.0
Aflatoxin B1 46
Aflatoxin B2 35
Aflatoxin M2 29
Aflatoxin G1 27
Aflatoxin G2 6
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
AFM1 Clean Up Reagent (Optional)
Warnings and Precautions
The standards contain Aflatoxin M1 . Handle with particular care.
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or lots except for components with the same part No’s within their expiration dates.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulation material under the assay plates during incubation.
Make sure you are using distilled or deionized water since water quality is important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards first and then your samples.
Add standards to plate only in the order from low concentration to high concentration as this will minimize the risk of compromising the standard curve.
Always refrigerate plates in sealed bags with a desiccant to maintain stability. Prevent condensation from forming on plates by allowing them equilibrate to room temperature (20 – 25C / 68 – 77F) while in the packaging.
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