| Trade - Want to Buy |
| Topic | Gentamicin ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
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| Country | USA |
| Date Created | 9/28/2013 12:04:13 PM |
| Message | Product Description
REAGEN™Gentamicin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine. The unique features of the kit are:
High recovery (80-105%), rapid (10 - 40 minutes), and cost-effective extraction methods.
High sensitivity (0.08 ng/g or ppb)
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
Analytes Cross-Reactivity (%)
Gentamicin 100
Neomycin <0.1
Kanomycin <0.1
Streptomycin <0.1
Tobramycin <0.1
Amikazin <0.1
Dihydrostreptomycin <0.1
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Gentamicin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Gentamicin-coated Plate 1x 96-well Plate (8 wells x 12 strips) 2-8C
Gentamicin Standards:
Negative control (white cap tube)
0.08 ng/mL (yellow cap tube)
0.32 ng/mL (orange cap tube)
1.25 ng/mL (pink cap tube)
5.0 ng/mL (purple cap tube)
20 ng/mL (blue cap tube)
50 ng/mL (spiking, optional, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
2.0 mL 2-8C
Gentamicin Antibody #1 12 mL 2-8C *
Antibody #2 18 mL 2-8C
10X Sample Extraction Buffer 25 mL 2-8C
20X Wash Solution 30 mL 2-8C
Stop Buffer 14 mL 2-8C
TMB Substrate 10 mL 2-8C
8x embryonic egg buffer(optional) 20ml 2-8C
If you are not planning to use the kit for over 3 months, store Gentamicin Antibody #1 and 1X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Embryonic egg 4
Egg 5
Feed 6.25
Honey 6.25
Meat/liver/kidney 2.5
Milk 2.5
Urine, Serum 5
PBS solution 0.08
Specificity
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
1M NaOH
pH test strips
Trichloroacetic acid (3% in water).
Vortex mixer (e.g. Genie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Warnings and Precautions
The standards contain gentamicin. Handle with particular care.
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or lots except for components with the same part No’s within their expiration dates. ANTIBODIES AND PLATES ARE KIT-AND LOT-SPECIFIC. Make sure that the antibody #2 and diluent are mixed in correct volumes.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulation material under the assay plates during incubation.
Make sure you are using only distilled or deionized water since water quality is very important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards first and then your samples.
Add standards to plate only in the order from low concentration to high concentration, as this will minimize the risk of compromising the standard curve.
Always refrigerate plates in sealed bags with a desiccant to maintain stability. Prevent condensation from forming on plates by allowing them equilibrate to room temperature (20 – 25C / 68 – 77F) while in the packaging.
SAMPLE PREPARATION
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