| Trade - Want to Buy |
| Topic | ofloxacin ELISA Test Kit |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | USA |
| Date Created | 9/28/2013 11:53:00 AM |
| Message | Product Description
REAGEN™ofloxacin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect ofloxacin to as low as 0.1 ppb level in various sample types and to satisfy customer concerns about food safety. The features of the kit are:
The extraction of ofloxacin from meat/tissue with a high recovery rate.
A quick ELISA assay (less than 1.5 hours regardless of number of samples).
High reproducibility.Procedure Overview
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The ofloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the ofloxacin-horseradish peroxidase (Ofloxacin-HRP) conjugate. If the ofloxacin residue is present in the sample, it will compete for the ofloxacin antibody, thereby preventing the Ofloxacin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the ofloxacin residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ ofloxacin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
0floxacinAb-coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8°C
Ofloxacin Standards:
Negative control (white cap tube)
0.1 ng/mL (yellow cap tube)
0.25 ng/mL (orange cap tube)
0.5 ng/mL (pink cap tube)
1.0 ng/mL (purple cap tube)
5.0 ng/mL (blue cap tube)
100 ng/mL (spiking, optional, red cap tube)
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
2-8°C
Ofloxacin-HRP 12 mL 2-8°C
10X Sample Extraction Buffer (Optional) 25 mL
20X Wash Solution ** 28 mL
Stop Buffer ** 14 mL
TMB Substrate ** 12 mL
Meat Extraction Buffer I (Optional) 5 mL
Meat Extraction Buffer II (Optional) 5 mL
Sample Balance Buffer (Optional) 25 mL
*If you are not planning to use the kit for over 3 months, store Ofloxacin-HRP at -20°C preferably or in a freezer.
Sensitivity (Detection Limit)
Sample Type Detection Limit (ng/g or ppb)
Meat/Liver/Kidney/Fish/Shrimp 0.1 (Organic Solvent Method); 2 (Rapid Method)
Specificity (Cross-Reactivity)
Analytes Cross-Reactivity (%)
Ofloxacin 100
Ciprofloxacin 82
Enrofloxacin 80
Danofloxacin 42
Enoxacin 14
Pipemidic acid 11
Benofloxacin 3
Oxolin acid 4
Nalidixic acid 2
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Warnings and Precautions
The standards contain ofloxacin. Handle with particular care.
Do not use the kit past the expiration date.
Do not mix reagents from different kits or lots except for components with the same part No’s within their expiration dates. HRP CONJUGATES AND PLATES ARE KIT-AND LOT-SPECIFIC.
Try to maintain a laboratory temperature of 20–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulation material under the assay plates during incubation.
Make sure you are using only distilled or deionized water since water quality is very important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards first and then your samples.
Add standards to plate only in the order from low concentration to high concentration as this will minimize the risk of compromising the standard curve.
Always refrigerate plates in sealed bags with a desiccant to maintain stability. Prevent condensation from forming on plates by allowing them equilibrate to room temperature (20–25°C / 68–77°F) while in the packaging.
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