| Trade - Want to Buy |
| Topic | Melamine ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | USA |
| Date Created | 9/28/2013 11:33:42 AM |
| Message | Product Description
REAGEN™Melamine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of melamine in milk, milk powder, infant formula, meat, fish, feed, chocolate and other food samples. The unique features of the kit are:
High recovery (80-105%),rapid(10-40 minutes),and cost-effective extraction methods.
High sensitivity(5ng/g or ppb)and low detection limit for liquid milk (0.055ppm).
High reproducibility.
A quick ELISA assay(less than 2 hours regardless of number of samples).
Procedure Overview
REAGEN™The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The enzyme-conjugate, will bind with the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Melamine ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Melamine Coated Plate 1 x 96-well Plate
(8 wells x 12 strips) 2-8°C
Melamine Standards:
Negative control (white cap tube)
5 ng/mL (yellow cap tube)
20 ng/mL (orange cap tube)
50 ng/mL (pink cap tube)
100 ng/mL (purple cap tube)
200 ng/mL (blue cap tube)
10 µg/mL (spiking, optional, red cap tube)
0.8mL
0.8mL
0.8mL
0.8mL
0.8mL
0.8mL
0.8mL
2-8°C
Melamine Antibody 12 mL 2-8°C
100X HRP Conjugate 250 µL
HRP Conjugate Diluent 20 mL
20X Wash Solution 28 mL
Stop Buffer 14 mL
TMB Substrate 12 mL
10 X Melamine Sample Extraction Buffer 20 mL X 2
1 X Melamine Clean Up Buffer 20 mL
1X Melamine Sample Balance Buffer 20 mL
10X Melamine Sample Dilution Buffer 14 mL
*If you are not planning to use the kit for over 1 month, store Melamine Standards, Melamine Antibody and 100X HRP Conjugate at -20°C or in a freezer.
Sensitivity (Detection Limit)
Sample Type Detection Limit (g/g or ppb)
Feed 0.055ppm
Meat/Fish 0.055ppm
Milk 0.055ppm
Milk Powder/Infant Formula 0.4ppm
Chocolate 0.4 ppm
Specificity (Cross-Reactivity)
Analytes Cross-Reactivity (%)
Melamine 100
Cyanurate 15
Ammeline 5
Ammelide <1
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Vortex mixer (e.g. Genie Vortex mixer from VWR)
10, 20, 100 and 1000 µL pipettes
Multi-channel pipette: 50-300 µL (Optional)
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