| Trade - Want to Buy |
| Topic | Nitroimidazole ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
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| Country | USA |
| Date Created | 9/28/2013 11:28:50 AM |
| Message | Product Description
REAGEN™Nitroimidazole ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Nitroimidazole in feed, meat (beef, chicken and pork), eggs, honey.
The unique features of the kit are:
High recovery (70% - 95%), and cost-effective extraction methods for various samples.
A quick ELISA assay (less than 1.5 hours regardless of number of samples).
High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The Dimetridazole antibody has been coated in the plate wells. During the analysis, sample is added along with the Dimetridazole -horseradish peroxidase (HRP) conjugate. If the target is present in the sample, it will compete for the Dimetridazole antibody, thereby preventing the Dimetridazole- HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of substrate((TMB), has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Nitroimidazole ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Dimetridazole -coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
Dimetridazole Standards:
Negative control
0.5ng/mL
1.5 ng/mL
3 ng/mL
6 ng/mL
12 ng/mL
100ng/mL (spiking, optional)
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL 2-8C
500x HRP-Conjugated 60 L 2-8C *
HRP Conjugate Diluent 15 mL 2-8C
TMB Substrate 12mL 2-8C
20X Wash Solution ** 28 mL 2-8C
Stop Buffer ** 14 mL 2-8C
Concentrate of Sample Extraction Buffer 30 g 2-8C
Sample Dilution Buffer 20 mL x 2 2-8C
* If you are not planning to use the kit for over 1 months, store 500 x HRP-Conjugated at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Meat/Liver/Kidney/Fish 0.5
Shrimp 0.5
Specificity
Analytes Cross-Reactivity (%)
Dimetridazole 100
Metronidazole 100
HydroxyNitroimidazole 32
Ronidazole 14
Ipronidazole 11
Nicarbazin 7
Halofuginone 3
Diclazuril 4
Robenidine 2
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Rotary evaporator or nitrogen gas
oscillator
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Acetonitrile
n-hexane
deionized water
Warnings and Precautions
The standards contain Dimetridazole . Handle with particular care.
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or lots except for components with the same part No’s within their expiration dates. HRP AND PLATES ARE KIT- AND LOT-SPECIFIC. Make sure that the antibody #2 and diluent are mixed in correct volumes.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulation material under the assay plates during incubation.
Make sure you are using only distilled or deionized water since water quality is very important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards first and then your samples.
Add standards to plate only in the order from low concentration to high concentration as this will minimize the risk of compromising the standard curve.
Always refrigerate plates in sealed bags with a desiccant to maintain stability. Prevent condensation from forming on plates by allowing them equilibrate to room temperature (20 – 25C / 68 – 77F) while in the packaging.
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