| Trade - Want to Buy |
| Topic | Neomycin ELISA Test Kit |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/27/2013 2:50:42 PM |
| Message | Product Description
REAGEN™Neomycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Neomycin in meat/liver/kidney.
The unique features of the kit are:
High recovery (80-105%), rapid (10 - 40 minutes), and cost-effective extraction methods.
High sensitivity (1 ng/g or ppb).
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Neomycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Neomycin-coated Microtiter Plate 1 x 96-well Plate 2-8C
NeomycinStandards*:
Negative control (white cap tube)
1ng/mL (orange cap tube)
2.5 ng/mL (pink cap tube)
5ng/mL (purple cap tube)
10 ng/mL (blue cap tube)
25 ng/mL green cap tube ()
1000 ng/mL (spiking, optional, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
2-8C
2-8C
Neomycin Antibody #1 12 mL 2-8C
1X HRP-Conjugated Antibody #2 12 mL
20X Wash Solution 30 mL
Stop Buffer 14 mL
TMB Substrate 10 mL
Sample Balance Buffer 14 mL
* If you are not planning to use the kit for over 3 months, store Neomycin Antibody #1 and 1X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Meat/liver/kidney 5
Specificity
Analytes Cross-Reactivity (%)
Neomycin 100
Gentamicin <0.1
Kanomycin <0.1
Streptomycin <0.1
Tobramycin <0.1
Amikazin <0.1
Dihydrostreptomycin <0.1
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Trichloroacetic acid (3% in water).
Vortex mixer (e.g. Genie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
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