| Trade - Want to Buy |
| Topic | Mabuterol ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
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| Country | USA |
| Date Created | 9/27/2013 2:40:36 PM |
| Message | Product Description
REAGEN™ mabuterol ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of mabuterol in feed, tissue(meat,liver and kidney), milk, serum,plasma and urine. The unique features of the kit are:
Rapid , high recovery (75 - 105%), and cost-effective extraction methods.
High sensitivity (0.05 ng/g or ppb) and low detection limit (0.025ppb for meat,0.25ppb for urine and 1ppb for feed) for various samples.
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ mabuterol ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
mabuterol-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
mabuterol Standards:
Negative control (white cap tube)
0.05 ng/mL (yellow cap tube)
0.15ng/mL (orange cap tube)
0.5 ng/mL (pink cap tube)
1.5 ng/mL (purple cap tube)
4.5 ng/mL (blue cap tube)
10 ng/mL (spiking, optional, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
2-8C
2-8C
mabuterol Antibody #1 15 mL 2-8C
100X HRP-Conjugated Antibody #2 250 L
Antibody #2 Diluent ** 20 mL
20X Wash Solution ** 30 mL
Stop Buffer ** 14 mL
TMB Substrate ** 10 mL
10xPBS
20X Sample Extraction Buffer
Sample Balance Buffer I (Optional)
Sample Balance Buffer II (Optional)
Liver Extraction Buffer (Optional) 25mL
10mL
10mL
62g
10mL
Beta-Agonist Clean Up buffer I(optional) 5mL
Beta-Agonist Clean Up buffer II(optional) 5mL
Beta-Agonist Clean Up buffer III(optioanal) 28mL
If you are not planning to use the kit for over 3 months, store mabuterol Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Feed 1
meat/chicken liver/kindey 0.025(methodI) 0.1(method II)
Swine/Bovine Liver and Kidney 0.1
Milk 0.25
Urine/Serum/Plasma 0.25
Specificity
Analytes Cross-Reactivity (%)
Mabuterol 100
Clenbuterol 76
Terbutaline 67
Mapenterol 55
Salbutamol 29
Cimaterol 26
Tolubuterol 23
Cimbuterol 11
Pirbuterol 2
Isoprenaline 1.2
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
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