| Trade - Want to Buy |
| Topic | Ractopamine ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/27/2013 2:33:51 PM |
| Message | Description
REAGEN™ Ractopamine ELISA Test Kit is a competitive enzyme immunoassay for this beta-agonist drug in meat, animal tissue, milk, feed, urine, serum and plasma. REAGEN™ Ractopamine ELISA Test Kit provides a unique extraction method, which is accurate, rapid, cost-effective and easy to use.
The features of the kit are:
The extraction of ractopamine from feed, meat, milk, tissue and urine can be finished in 10 - 60 minutes with a high recovery rate of 75 - 105%.
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
This kit is manufactured to the international quality standard ISO 9001:2008
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
Kit Contents Amount Storage
Ractopamine-coated Microtiter Plate 96-well plate(8 wells x 12 strips) 2-8C
Ractopamine Standards:
Negative control (white cap tube)
0.5 ng/mL (yellow cap tube)
1.0 ng/mL (orange cap tube)
5.0 ng/mL (pink cap tube)
10 ng/mL (purple cap tube)
25 ng/mL (blue cap tube)
100ng/mL(spiking, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL 2-8C
Ractopamine Antibody #1 15 mL 2-8C
100X HRP-Conjugated Antibody #2 250 L
Antibody #2 Diluent ** 20 mL
10X Sample Extraction Buffer ** 25 mL
20X Wash Solution ** 30 mL
Stop Buffer ** 20 mL
TMB Substrate ** 10 mL
* If you are not planning to use the kit for over 1 month, store Ractopamine Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Feed 0.5
Meat/Liver/Kidney 0.25
Milk 2.5
Urine 0.5
Specificity
Analytes Sensitivity (ppb) Cross-Reactivity (%)
Ractopamine 0.5 100
Ractopamine Glucuronide A 1.0 72
Ractopamine Glucuronide B 1.2 63
Ractopamine Glucuronide C 0.3 132
Clenbuterol >10,000 <0.01
Colterol >10,000 <0.01
Mabuterol >10,000 <0.01
Isoxsuprine 5,000 <0.01
Salmeterol 5,000 <0.01
Fenoterol >10,000 <0.01
Bitolterol >10,000 <0.01
Carbuterol >10,000 <0.01
Pirbuterol >10,000 <0.01
Terbuterol >10,000 <0.01
Timolol >10,000 <0.01
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Ethyl Acetate
Acetonitrile
n-Hexane
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