| Trade - Want to Buy |
| Topic | Olaquindox ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
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| Country | usa |
| Date Created | 9/27/2013 2:30:29 PM |
| Message | Product Description
REAGEN™Olaquindox ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Olaquindox in meat/fish/shrip and feed.
The unique features of the kit are:
High recovery (75% - 105%) .
Sensitivity:(1ppb)
High reproducibility.
A quick ELISA assay.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The Olaquindox has been coated in the plate wells. During the analysis, sample is added along with the Olaquindox antibody. If the Olaquindox residue is present in the sample, it will compete for the Olaquindox antibody, thereby preventing the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Olaquindox residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Olaquindox ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Olaquindox -Coated Plate 96-well plate (8 wells x 12 strips) 2-8C
Olaquindox Standards:
Negative control
1ng/mL
3ng/mL
9 ng/mL
27 ng/mL
81ng/mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL 2-8C
1000 ng/mL (spiking, optional) 1.0 mL 2-8C
Olaquindox- HRP Conjugate 7 mL
Olaquindox antibody 7 mL
20X Wash Solution 40 mL
Stop Buffer 7 mL
TMB A 7 mL
TMB B 7 mL
2X Sample Extraction Buffer 50 mL
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
meat/fish/shrip 1
Feed 100
Specificity
Analytes Cross-Reactivity (%)
Olaquindox 100
Carbadox <7
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Warnings and Precautions
The standards contain Olaquindox .Handle with particular care.
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or lots except for components with the same part No’s within their expiration dates. Olaquindox-HRP Conjugates and plates are kit and lot-specific.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulation material under the assay plates during incubation.
Make sure you are using only distilled or deionized water since water quality is very important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards first and then your samples.
Add standards to plate only in the order from low concentration to high concentration as this will minimize the risk of compromising the standard curve.
Always refrigerate plates in sealed bags with a desiccant to maintain stability. Prevent condensation from forming on plates by allowing them equilibrate to room temperature (20 – 25C / 68 – 77F) while in the packaging.
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