| Trade - Want to Buy |
| Topic | Chlortetracycline ELISA Test Kit |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
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| Country | USA |
| Date Created | 9/27/2013 2:29:23 PM |
| Message | Product Description
REAGEN™Chlortetracycline ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Chlortetracycline in hony, milk, meat and urine. The unique features of the kit are:
Rapid , high recovery (80-105%), and cost-effective extraction methods.
High sensitivity (0.05 ng/g or ppb) and low detection limit (2.5ppb for honey sample).
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Chlortetracycline ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Chlortetracycline-coated Microtiter Plate 1x 96-well plate(8wells x12 strips) 2-8C
Chlortetracycline Standards stock
(450ng powder)
Empty Vials for standards:
Negative control (white cap tube)
0.05 ng/mL (yellow cap tube)
0.15ng/mL (orange cap tube)
0.5 ng/mL (pink cap tube)
1.5 ng/mL (purple cap tube)
4.5 ng/mL (blue cap tube)
3
1
1
1
1
1
1 -20C
2-8C
Chlortetracycline Antibody #1 3xtubes 2-8C
Antibody #1 diluent 15ml
100X HRP-Conjugated Antibody #2 0.3mL
Antibody #2 Diluent ** 20 mL
20X Wash Solution ** 30 mL
Stop Buffer ** 20 mL
TMB Substrate ** 10mL
20Xtet meat extration buffer 20mL
* If you are not planning to use the kit for over 3 months, store Chlortetracycline Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Honey 2.5
Meat 10
Milk 10
Milk powder 45
Serum 2
Urine 2
Specificity
Analytes Cross-Reactivity (%)
Chlortetracycline 100
Oxytetracycline 91
Minocycline 73
Demeclocycine 39
Tetracycline 119
Doxycycline 60
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
10Mmpbs buffer: 0.24g KH2PO4 +1.44 g Na2HPO4+ 8gNaCl, + 0.2gKCl, adjust pH to 7.4 with NaOH, fill up to 1000 mL with distilled water
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