| Trade - Want to Buy |
| Topic | Tylosin ELISA Test Kit |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
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| Country | USA |
| Date Created | 9/27/2013 1:47:59 PM |
| Message | Product Description
REAGEN™ Tylosin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of tylosin in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine. The unique features of the kit are:
High recovery (80-105%), rapid (10 - 40 minutes), and cost-effective extraction methods.
High sensitivity (1.0 ng/g or ppb)
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
Tylosin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Tylosin-Coated Plate 1 x 96-well Plate (8 wells x 12 strips) 2-8C
Tylosin Standard stock: 125 ng (powder) 3 tubes 2-8C
Tylosin Antibody#1 15mL
100X HRP-Conjugated Antibody #2 250L
Antibody #2 Diluent ** 20 mL
10X Sample Extraction Buffer ** 25 mL
20X Wash Solution ** 30 mL
Stop Buffer ** 20 mL
TMB Substrate ** 10 mL
* If you are not planning to use the kit for over 1month, store Tylosin kit at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Bran Mash 10 (Rapid Method);
Bran Mash 2.5 (Organic Extraction Method)
Egg 10 (Rapid Method);
Egg 2.5 (Organic Extraction Method)
Honey 2
Milk 10
Meat/Liver/Kidney 10
Urine 20
Specificity
Analytes Cross-Reactivity (%)
Tylosin 100.0
Spyramicin 8
Tilmycosin < 0.05
Tetracyclines < 0.01
Gentamicin < 0.01
Ampicillin < 0.01
Florfenicol < 0.01
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Acetonitrile
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