| Trade - Want to Buy |
| Topic | Aminoglycosides ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
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| Country | usa |
| Date Created | 9/27/2013 1:46:54 PM |
| Message | Product Description
REAGEN™Aminoglycosides ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aminoglycosides and dihydroAminoglycosides in feed, honey, kidney, liver, meat (beef, chicken and pork), milk, serum and urine. The unique features of the kit are:
Rapid (10 - 30 minutes), and organic reagent-free extraction methods for various samples with high recovery (80 - 105%).
High sensitivity (0.5 ng/g or ppb) and low detection limit (5 ng/g or ppb for meat and milk).
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Aminoglycosides ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Aminoglycosides-coated Microtiter Plate 96well plate (8 wells x 12 strips) 2-8C
Aminoglycosides Standards*:
Negative control (white cap tube)
0.5 ng/mL (yellow cap tube)
1.0 ng/mL (orange cap tube)
2.5 ng/mL (pink cap tube)
5.0 ng/mL (purple cap tube)
10 ng/mL (blue cap tube)
1000ng/mL (spiking, optional, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
2-8C
Aminoglycosides Antibody #1 15 mL 2-8C
100X HRP-Conjugated Antibody #2 250 L
Antibody #2 Diluent 20 mL
20X Wash Solution 30 mL
Stop Buffer 20 mL
TMB Substrate 10mL
Sample Balance Buffer
10X PBS
200X Sample Extraction Buffer 0.5 mL
25 mL
25 mL
If you are not planning to use the kit for over 3 months, store Aminoglycosides Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Feed 5
Honey 10
Meat/Liver/Kidney 5
Milk 10
Milk Powder 25
Serum 5
Urine 5
Specificity
Analytes Cross-Reactivity (%)
Aminoglycosides 100
Streptomycin 100
Neomycin 98
Kanamycin 84
Gentamicin 75
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
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