| Trade - Want to Buy |
| Topic | Lincomycin ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
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| Country | usa |
| Date Created | 9/27/2013 1:43:17 PM |
| Message | Product Description
REAGEN™ Lincomycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Lincomycin in meat,fish,shrimp,honey and urine samples. The unique features of the kit are:
High recovery (80-105%),rapid(less than 40 minutes) extraction methods for various samples.
High sensitivity (0.2ng/g or ppb).
High reproducibility..
A quick ELISA assay(less than 1 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Lincomycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused micro-wells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Lincomycin Coated Plate 1x 96-well Plate(8wells x12 strips) 2-8°C
Lincomycin Standards:
Negative control (white cap tube)
0.2ng/mL(yellow cap tube)
0.6 ng/mL(orange cap tube)
1.8 ng/mL (pink cap tube)
5.4 ng/mL(purple cap tube)
16.2 ng/mL(blue cap tube)
1000 ng/mL (spiking, red cap tube)
8.0 ng/mL (blue cap tube)
100 µg/mL (spiking, optional, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
2-8°C *
Lincomycin Antibody #1 7 mL 2-8°C
1X HRP Conjugate Antibody #2 7 mL
20X Wash Solution 38 mL
Stop Buffer 7 mL
TMB Substrate 12 mL
2X Sample Dilution Buffer 48 mL
* If you are not planning to use the kit for over 1 month, store Lincomycin
Standards, Lincomycin Antibody and 1X HRP Conjugate at -20°C or in a freezer.
Sensitivity (Detection Limit)
Sample Type Detection Limit (ppb)
Meat/Fish/shrimp 4.0
Honey 1.0
Urine 2.0
Specificity (Cross-Reactivity)
Analytes Cross-Reactivity (%)
Lincomycin 100
Spiramycin < 0.1
Erythrocin < 0.1
Kanamycin < 0.1
Tilmicosin < 0.1
Tylosin < 0.1
Required Materials Not Provided With the Kit
1. Microtiter plate reader (450 nm)
2. Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
3. Vortex mixer (e.g. Genie Vortex mixer from VWR)
4. 10, 20, 100 and 1000 µL pipettes
5. Multi-channel pipette: 50-300 µL (Optional)
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