| Trade - Want to Buy |
| Topic | Monensin ELISA Test Kit |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | USA |
| Date Created | 9/27/2013 1:35:21 PM |
| Message | Product Description
REAGEN™ Monensin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect monensin in feed to satisfy customer concerns about food safety. The Monensin ELISA Test Kit provides a unique extraction method, which is accurate, rapid, cost effective and easy to use. The features of the kit are:
The extraction of monensin from samples can be finished in 45 -60 minutes with a high recovery rate of 75 - 105%
High reproducibility
A quick ELISA assay (less than 1.5 hours regardless of number of samples)
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The monensin antibody has been coated in the plate wells. During the analysis, sample is added along with the monensin-horseradish peroxidase conjugate. If monensin residue is present in the sample, it will compete for the monensin antibody, thereby preventing the monensin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the monensin residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Monensin ELISA Test Kit has the Cyproheptadineacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Monensin Ab -coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
Monensin Standard Stock 300 ppb 1.0 mL
4x Monensin Sample Buffer 30 mL
Monensin-HRP Conjugate 12 mL 2-8C
Monensin Clean up Mix 26 g
20X Wash Solution ** 30mL
Stop Buffer ** 14 mL
TMB Substrate ** 10 mL
* If you are not planning to use the kit for over 1 month, store Monensin-HRP Conjugate at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Feed 30
Specificity
Analytes Cross-Reactivity (%)
Monensin 100
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Vortex mixer (e.g. Genie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Methanol
Analytical balance
Multi-channel pipette: 50-300 L (Optional)
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