| Trade - Want to Buy |
| Topic | Avermectins/ Ivermectin ELISA Test Kit |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/27/2013 1:33:02 PM |
| Message | Product Description
REAGEN™ Avermectins/ Ivermectin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Abamectin in meat, animal tissue, Vegetables and fruit.,
The unique features of the kit are:
The extraction of Avermectins from milk can be finished with a high recovery rate of 75 - 105%.
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Avermectins/ Ivermectin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Avermectin-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
Ivermectin Standards:
Negative control
0.5 ng/mL
1.0 ng/mL
2 ng/mL
4 ng/mL
8 ng/mL
1200 ng/mL (spiking, optional)
1.0mL
1.0mL
1.0mL
1.0mL
1.0mL
1.0mL
1.0mL 2-8C *
Avermectin Antibody #1 12ml 2-8C
100X HRP-Conjugated Antibody #2 250 L
Antibody #2 Diluent 20 mL
20X Wash Solution 30mL
Stop Buffer 14 mL
TMB Substrate 10 mL
Avermectin Clean Up Mix
Milk Extraction Buffer I
Milk Extraction Buffer II
10X Sample Extraction Buffer 25g
10 mL
28 mL
25mL
* If you are not planning to use the kit for over 1 month, store Ivermectin Standard , Avermectin Antibody #1, 100X HRP-Conjugated Antibody #2 at -20C preferably or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Meat/Fish/Shrimp 5
Milk 5
Urine 5
Water 5
Specificity
Molecules Cross-Reactivity (%)
Ivermectin 100%
Emamectin 100%
Abamectin 113%
Eprinomectin 100%
Doramectin <1%
Moxidectin <1%
Milbamycin <1%
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Acetonitrile ，ethanol
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