| Trade - Want to Buy |
| Topic | Ethoxyquin ELISA Test Kit |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
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| Country | USA |
| Date Created | 9/27/2013 1:22:48 PM |
| Message | Product Description
REAGEN™ Ethoxyquin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of ethoxyquin in fish, shrimp, feed and meat. Ethoxyquin is a quinoline-based antioxidant used as a food preservative and a pesticide. To date, the U.S. Food and Drug Administration (FDA) has only found a verifiable connection between ethoxyquin and buildup of protoporphyrin IX in the liver, as well as elevations in liver-related enzymes in some animals, but there are no known health consequences from these effects. In Japan, the ethoxyquin content must not be higher than 10 ppb in shrimp, 1 ppm in fish.
The REAGEN™thoxyquin ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect ethoxyquin in fish, shrimp to satisfy customer’s concerns about food safety. The unique features of the kit are:
High recovery (>75%) and cost-effective extraction methods
High sensitivity (0.1 ng/g or ppb)
Quick ELISA assay (less than 2 hours regardless of the number of samples)
High reproducibility
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The ethoxyquin conjugate has been coated in the plate wells. During the analysis, a sample is added along with the primary antibody specific for ethoxyquin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the ethoxyquin attached to the wells. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody associated with the ethoxyquin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Ethoxyquin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Ethoxyquin Coated Plate 1 x 96-well Plate (8 wells x 12 strips) 2-8°C
Ethoxyquin Standard Stock: 1000 ppb 0.8 mL
-20°C *
10X Ethoxyquin Sample Suspension Solution 14 mL 2-8°C *
Ethoxyquin Antibody #1 12 mL 2-8°C *
100X HRP-Conjugated Antibody #2 250L 2-8°C
Antibody #2 Diluent 20 mL 2-8°C
Ethoxyquin Clean Up Mix 12.5g 2-8°C
20X Wash Solution 28 mL 2-8°C
Stop Buffer 14 mL 2-8°C
TMB Substrate 12 mL 2-8°C
*If you are not planning to use the kit for over 1 month, store the Ethoxyquin Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20 C or in a freezer.
Sensitivity (Detection Limit)
Sample Type Detection Limit (ppb)
feed 1
Fish/Meat 1
Shrimp 1
Specificity (Cross-Reactivity)
Analytes Cross-Reactivity (%)
Ethoxyquin 100
Enrofloxacin <0.1
Ciprofloxacin <0.1
Danofloxacin <0.1
Norfloxacin <0.1
Enoxacin <0.1
Pipemidic acid <0.1
Ofloxacin <0.1
Benofloxacin <0.1
Flumequin <0.1
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Vortex mixer (e.g. Genie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Evaporator (Optional)
MgSO4.
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