| Trade - Want to Buy |
| Topic | Chloramphenicol (CAP) ELISA Test Kit |
| Name | nicole |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | usa |
| Date Created | 9/27/2013 1:22:00 PM |
| Message | Product Description
REAGEN™Chloramphenicol (CAP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine.
The unique features of the kit are:
High recovery (80-105%), rapid (10 - 40 minutes), and cost-effective extraction methods.
High sensitivity (0.05 ng/g or ppb) and low detection limit (0.025 ng/g or ppb) for shrimp, fish and meat samples.
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The CAP antibody has been coated in the plate wells. During the analysis, sample is added along with the CAP-horseradish peroxidase conjugate. If the CAP residue is present in the sample, it will compete for the CAP antibody, thereby preventing the CAP-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the CAP residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Chloramphenicol (CAP) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
CAP Antibody-Coated Plate 1x96-well Plate (8wells x12strips) 2-8C
CAP Standards:
Negative control (white cap tube)
0.05 ng/mL (yellow cap tube)
0.15 ng/mL (orange cap tube)
0.5 ng/mL (pink cap tube)
1.5 ng/mL (purple cap tube)
4.5 ng/mL (blue cap tube)
10ng/mL(spiking, optional, red cap tube)
2.0 mL
2.0 mL
2.0 mL
2.0 mL
2.0 mL
2.0 mL
2.0 mL
2-8C
CAP-HRP Conjugate 8 mL 2-8C *
10X Sample Extraction Buffer ** 25 mL 2-8C
20X Wash Solution ** 30 mL 2-8C
Stop Buffer ** 20 mL 2-8C
TMB Substrate ** 10 mL 2-8C
* If you are not planning to use the kit for over 3 months, store CAP-HRP Conjugate at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Feed 0.25
Fish/Shrimp/Meat 0.025
Eggs 0.05
Honey 0.05
Milk 0.25
Milk powder 0.1
Serum 0.04
Serum 0.5 (rapid extraction)
Urine 0.04
Urine 0.05 (rapid extraction)
Specificity
Analytes Cross-Reactivity (%)
Chloramphenicol (CAP) 100.0
Chloramphenicol Glucuronide 76.8
Chloramphenicol base <0.5
Thiamphenicol < 0.05
Tetracyclines < 0.01
Gentamicin < 0.01
Ampicillin < 0.01
Florfenicol < 0.01
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Ethyl acetate
n-Hexane
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