| Trade - Want to Buy |
| Topic | Tetracycline(TET) ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | usa |
| Date Created | 9/27/2013 1:21:03 PM |
| Message | Product Description
REAGEN™ Tetracycline (TET) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Tetracycline in honey, milk, urine, meat, fish and shrimp.
The unique features of the kit are:
High recovery (80-105%), rapid (less than 30 minutes), and cost-effective extraction methods.
High sensitivity (0.05 ng/g or ppb) and low detection limit (e.g. 2.5 ng/g or ppb for honey samples).
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Tetracycline (TET) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Tetracycline-coated Plate 1 x 96-well Plate (8 wells x 12 strips) 2-8C
Tetracycline Standard Stock (Powder)
Empty Vials for Standards:
Negative control (white CAP tube)
0.05 ng/mL (yellow CAP tube)
0.15 ng/mL (orange CAP tube)
0.3 ng/mL (pink CAP tube)
0.6 ng/mL (purple CAP tube)
1.5 ng/mL (blue CAP tube) 3 x 225 ng
1
1
1
1
1
1 -20C
2-8C
Tetracycline Antibody #1 (powder) 3 X tubes 2-8C
Antibody#1 Diluent 15 mL
100X HRP-Conjugated Antibody #2 0.3 mL
Antibody #2 Diluent ** 20 mL
20X Wash Solution ** 30 mL
Stop Buffer ** 14 mL
TMB Substrate** 10 mL
20X TET Meat Extraction Buffer
(optional, for meat sample only, ask from local distributor) 20 mL
*If you are not planning to use the kit for over 1 month, storing Tetracycline Standard Stock, Tetracycline Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer is recommended.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Honey 2.5
Meat/Fish/Shrimp 10
Milk 10
Milk powder 45
Serum 2
Urine 2
Specificity
Analytes Cross-Reactivity (%)
Tetracycline (TET) 100.0
Chlortetracycline 98
Rolitetracycline 113
Minocycline 122
Demeclocycline 37
Oxytetracycline 12
Doxycycline 6
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Vortex mixer, (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
10 mM PBS buffer: 0.24 g KH2PO4 + 1.44 g Na2HPO4 + 8 g NaCl, + 0.2 g KCl, adjust pH to 7.4 with NaOH, fill up to 1000 mL with distilled water
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