| Trade - Want to Buy |
| Topic | Salbutamol ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/27/2013 1:20:00 PM |
| Message | Product Description
REAGEN™ Salbutamol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine. The unique features of the kit are:
Rapid (10 - 30 minutes) extraction method for various samples with high recovery (75 - 105%).
High sensitivity (0.05 ng/g or ppb) and low detection limit (0.025 ng/g or ppb for meat/tissue, 0.05 ng/g or ppb for urine and 0.2 ng/g or ppb for animal feed).
High reproducibility.
A quick ELISA assay.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Salbutamol ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Salbutamol -coated Plate 1 x 96-well (8 wells x 12 strips) 2-8C
Salbutamol Standards:
Negative control (white cap tube)
0.05 ng/mL (yellow cap tube)
0.15 ng/mL (orange cap tube)
0.5 ng/mL (pink cap tube)
1.5 ng/mL (purple cap tube)
4.5 ng/mL (blue cap tube)
10 ng/mL (spiking, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
2-8C
Salbutamol Antibody #1 15 mL 2-8C
100X HRP-Conjugated Antibody #2 250 L
Antibody #2 Diluent ** 20 mL
20X Wash Solution ** 30 mL
Stop Buffer ** 20 mL
TMB Substrate ** 10 mL
Sample Balance Buffer **
10X PBS **
200X Sample Extraction Buffer ** 0.5 mL
25 mL
25 mL
If you are not planning to use the kit for over 3 months, store Salbutamol Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Feed 0.2 (Rapid Extraction Method)
Honey 1.0
Meat/Liver/Kidney 0.025 (Organic Reagent Extraction Method)
Milk 0.25
Urine 0.05
Specificity
Analytes Cross-Reactivity (%)
Salbutamol 100
Clenbuterol 60
Mapenterol 12
Tolubuterol 6.8
Terbutaline 1.4
Cimbuterol 0.7
Mabuterol 0.5
Cimaterol 0.1
Pirbuterol <0.1
Isoprenaline <0.1
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
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