| Trade - Want to Buy |
| Topic | Trifluralin ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
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| Country | USA |
| Date Created | 9/27/2013 1:18:57 PM |
| Message | Product Description
REAGEN™ Trifluralin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of trifluralinl in fish, shrimp and meat.
The unique features of the kit are:
High recovery (75-105%), rapid (10 - 40 minutes), and cost-effective extraction methods .
High sensitivity (0.25 ng/g or ppb).
Quick ELISA assay (less than 2 hours regardless of the number of samples).
High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The trifluralin conjugate has been coated in the plate wells. During the analysis, a sample is added along with the primary antibody specific for trifluralin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the trifluralin attached to the wells. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody associated with the trifluralin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Trifluralin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Trifluralin Coated Plate 1x96-well Plate (8wells x12strips) 2-8C
Trifluralin Standards:
1,500 ppb standard stock
1.0 mL -20C
10X Trifluralin Sample Suspension Solution 20 ml 2-8C *
Trifluralin Clean Up Mix 10 g x 2 2-8C
Trifluralin Antibody #1 (powder) 3 bottles 2-8C
100X HRP-Conjugated Antibody #2 250 µL 2-8C
Antibody #2 Diluent 20 mL 2-8C
20X Wash Solution 30 mL 2-8C
Stop Buffer 20 mL 2-8C
TMB Substrate 10 mL 2-8C
* If you are not planning to use the kit for over 3 months, store trifluralin Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Fish/Meat 0.4
Shrimp 0.4
Specificity
Analytes Cross-Reactivity (%)
Trifluralin 100
Alachlor <0.1
Atrazine <0.1
Bromacil <0.1
Butachlor <0.1
Metolachlor <0.1
Prometon <0.1
Simazine <0.1
Alpha-Chlordane <0.1
Endrin <0.1
Gamma-Chlordane <0.1
Heptachlor <0.1
Heptachlor Epoxide Isomer B <0.1
Hexachlorobenzene <0.1
Hexachlorocyclopentadine <0.1
Methoxychlor <0.1
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Evaporator (Optional)
MgSO4.
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