| Trade - Want to Buy |
| Topic | Furazolidone (AOZ) ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/27/2013 1:03:26 PM |
| Message | Product Description
REAGEN™Furazolidone (AOZ) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of furazolidone in feed, fish, shrimp, meat (beef, chicken and pork), eggs, honey, milk, serum and urine. The unique features of the kit are:
Rapid (4 hours), high recovery (80 - 105%), and cost-effective extraction methods for various samples.
High sensitivity (0.005 ng/g or ppb) and low detection limit (0.01 ng/g or ppb) for various samples.
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
Furazolidone (AOZ) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
AOZ-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
AOZ Standards:
Negative control (white cap tube)
0.005 ng/mL (yellow cap tube)
0.015 ng/mL (orange cap tube)
0.05 ng/mL (pink cap tube)
0.15 ng/mL (purple cap tube)
0.5 ng/mL (blue cap tube)
10 ng/mL (spiking, optional, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
2-8C
AOZ Antibody #1 12 mL 2-8C
100X HRP-Conjugated Antibody #2 250 L
Antibody #2 Diluent ** 20 mL
20X Wash Solution ** 30 mL
Stop Buffer ** 20 mL
TMB Substrate ** 10 mL
50 mM 2-Nitrobenzaldehyde **
Liver Extration buffer(option)
Sample balance buffer I(option)
Sample balance buffer II(option)
10X Sample Extraction Buffer ** 1.8 mL
10ml
10ml
62g
25 mL
* If you are not planning to use the kit for over 3 months, store AOZ Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Shrimp/Fish/Meat 0.01
Egg/Egg Powder 0.01
Honey 0.01
Serum 0.01
Urine 0.01
Feed 0.02
Milk 0.01
Specificity
Analytes Cross-Reactivity (%)
AOZ 100
AMOZ <0.02
AHD < 0.01
SEM < 0.01
Required Materials Not Provided With the Kit
1. Microtiter plate reader (450 nm)
2. Incubator
3. Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
4. Rotary evaporator or nitrogen gas
5. Vortex mixer (e.g. Gneie Vortex mixer from VWR)
6. 10, 20, 100 and 1000 L pipettes
7. Multi-channel pipette: 50-300 L (Optional)
8. Ethyl acetate
9. 0.1 M K2HPO4
10. n-Hexane (or n-Heptane)
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