| Trade - Want to Buy |
| Topic | Nitrofurantoin(AHD) ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/27/2013 12:46:07 PM |
| Message | Product Description
REAGEN™Nitrofurantoin (AHD) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of nitrofurantoin in feed, fish, shrimp, meat (beef, chicken and pork), eggs, honey, milk, serum and urine.
The unique features of the kit are:
Rapid (4 hours), high recovery (75% - 105%), and cost-effective extraction methods for various samples.
High sensitivity (0.025 ng/g or ppb) and low detection limit (0.05 ng/g or ppb) for various samples.
High reproducibility.
A quick ELISA assay (less than 1.5 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The AHD antibody has been coated in the plate wells. During the analysis, sample is added along with the AHD-horseradish peroxidase (AHD-HRP) conjugate. If the AHD residue is present in the sample, it will compete for the AHD antibody, thereby preventing the AHD-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the AHD residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Nitrofurantoin (AHD) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
AHD Antibody-Coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
AHD Standards:
Negative control (white cap tube)
0.025 ng/mL (yellow cap tube)
0.1 ng/mL (orange cap tube)
0.25 ng/mL (pink cap tube)
0.75 ng/mL (purple cap tube)
2.5 ng/mL (blue cap tube)
10 ng/mL (spiking, optional, red cap tube)
2.0 mL
2.0 mL
2.0 mL
2.0 mL
2.0 mL
2.0 mL
2.0 mL
2-8C
2-8C
AHD- HRP Conjugate 6 mL 2-8C
10X Sample Extraction Buffer ** 25 mL
20X Wash Solution ** 30 mL
Stop Buffer ** 20 mL
TMB Substrate ** 10 mL
50 mM 2-Nitrobenzaldehyde ** 1.8 mL
* If you are not planning to use the kit for over 1 month, store AHD- HRP Conjugate at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Feed 0.1
Fish/Shrimp/Meat 0.05
Egg/Egg Powder 0.05
Honey 0.05
Milk 0.05
Serum 0.05
Urine 0.05
Specificity
Analytes Cross-Reactivity (%)
AHD 100
AOZ <0.04
SEM < 0.06
AMOZ < 0.05
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Ethyl acetate
0.1 M K2HPO4
n-Hexane (or n-Heptane)
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