| Trade - Want to Buy |
| Topic | Cimaterol ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
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| Country | USA |
| Date Created | 9/27/2013 12:45:09 PM |
| Message | Product Description
REAGEN™Cimaterol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of cimaterol in feed, meat/tissue, milk and urine. Cimaterol can improve the meat/fat ratio in fattened animals and accelerate livestock growth. They also have a relaxing effect on non-striated musculature and can be used as antiasthmatic and tocolytic agents in human beings. Therefore, excess cimaterol residues in livestock products may lead to health risks for consumers. This has led to a prohibition of cimaterol use in food production. The amount of cimaterol residues can be determined by methods such as HPLC or GC-MS. However, these traditional methods usually require very expensive preparation procedures. Alternatively, enzyme immunoassay (ELISA) can be used as a screening system, which is simple, rapid, sensitive and cost-effective compared with traditional methods.
The unique features of the kit are:
Rapid (10 - 30 minutes) extraction method for various samples with high recovery (75 - 95%).
High sensitivity (0.25 ng/g or ppb) and low detection limit (0.125 ng/g or ppb for meat/Chicken Liver, 1.25 ng/g or ppb for urine, and 2.5 ng/g or ppb for feed).
A quick ELISA assay (less than 2 hours regardless of number of samples).
High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
Kit Contents Amount Storage
Cimaterol-coated Microtiter Plate 1 x 96-well plate (8 x 12 strips) 2-8C
Cimaterol Standards:
Negative control (white cap tube)
0.25 ng/mL (yellow cap tube)
1.0 ng/mL (orange cap tube)
5.0 ng/mL (pink cap tube)
20 ng/mL (purple cap tube)
50 ng/mL (blue cap tube)
100 ng/mL(spiking, optional, red cap tube)
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL 2-8C
Cimaterol Antibody #1 12 mL 2-8C *
100X HRP-Conjugated Antibody #2 250 L
Antibody #2 Diluent 20 mL
20X Wash Solution 28 mL
TMB Substrate 12 mL
Stop Buffer 14 mL
Sample Balance Buffer I (Optional)
Sample Balance Buffer II (Optional)
Liver Extraction Buffer (Optional)
Beta-Agonist Clean Up Buffer I
Beta-Agonist Clean Up Buffer II
Beta-Agonist Clean Up Buffer III
10X PBS
20X Sample Extraction Buffer (Optional) 10 mL
62g
10 mL
5 mL
5 mL
28 mL
25 mL
10 mL
* If you are not planning to use the kit for over 1 month, store Cimaterol Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Feed 2.5
Meat/Chicken Liver 0.125 (Method I); 0.5 (Method II)
Swine/Bovine Liver and Kidney 0.5
Milk 1.25
Urine/Serum 1.25
Specificity
Analytes Cross-Reactivity (%)
Cimaterol 100
Clenbuterol 69
Mapenterol 15
Tolubuterol 12
Terbutaline 2.3
Mabuterol 0.5
Pirbuterol <0.1
Isoprenaline <0.1
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
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