| Trade - Want to Buy |
| Topic | No-Mel Milk Total Protein Assay Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/27/2013 12:44:11 PM |
| Message | Product Description
REAGEN™No-Mel Milk Total Protein Assay Kit is a colorimetric assay for the determination of protein in milk, powdered milk, cream, ice cream and chocolate drink.
The unique features of the kit are:
Rapid and simple method (modified version of AOAC method) less than 10 minutes.
Minimal sample prep.
Highly accurate and reproducible.
Not influenced by the presence of melamine in samples (<2 mg/mL in milk).
Procedure Overview
The No-Mel Milk Total Protein Assay Kitmeasures the changes in soluble dye concentration caused by the presence of protein in dairy samples. The color change caused by consumption of the dye (measured by the absorbance at 490 nm) is related to the amount of protein present in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™No-Mel Milk Total Protein Assay Kit has the capacity for 96 determinations ortesting of 42 samples in duplicate (using 12 wells for standards).The shelf life is 6 months when the kit is properly stored.
Kit Contents Amount Storage
Microtiter Plate 96-well plates (8 wells x 12 strips) Room Temperature
Calibration Standard:
(protein equivalent concentration):
Std 1:0% protein (white cap tube)
Std 2:1% protein (yellow cap tube)
Std 3:2.5% Protein(orange cap tube)
Std 4:2.8% protein (pink cap tube)
Std 5:3.1% protein(purple cap tube)
Std 6:4% protein (blue cap tube)
1.6ml
1.6ml
1.6ml
1.6ml
1.6ml
1.6ml Room Temperature
Dye Reagent 2 X 26ml Room Temperature
Dairy Sample Types
Homogenized Milk
Low-fat Milk
Milk Powder
Cream
Ice Cream
Chocolate Drink
Required Materials Not Provided With the Kit
Spectrophotometer (digital instrument preferred) or microtiter plate reader (with 490 nm absorbance filter)
1 cm cuvettes (glass, quartz or polystyrene) or microplate
Microcentrifuge tubes (polypropylene)
Microcentrifuge
Warnings and Precautions
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or different lots.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F).
Avoid exposure of dye reagents to light.
Make sure you are using only distilled or deionized water since water quality is very important.
When pipetting samples or reagents into an empty microtiter plate, place th
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