| Trade - Want to Buy |
| Topic | Urea Enzymatic Assay Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/27/2013 12:43:00 PM |
| Message | Product Description
REAGEN™ Urea Enzymatic Assay Kit is a icolorimetric assay for the determination of urea in liquid samples. Urea Enzymatic Assay Kit provides rapid, accurate, proven results even in complex liquid mixtures. The limit of detection for the test is 20 ppm urea for milk. The linear range of the assay is 20 – 1000 ppm analyte.
The unique features of the kit are:
Rapid and simple method
Minimal sample prep
High accurate and reproducible
Procedure Overview
REAGEN™ Urea Enzymatic Assay Kit measures the concentration of urea using the urease enzyme, which converts urea to ammonia.
urease
CO(NH2)2 + H2O CO2 + 2 NH3
The ammonia produced from the urea is then directly detected by a colorimetric chemical reaction.
Kit Contents, Storage and Shelf Life
REAGEN™ Urea Assay Kit has the capacity for 96 determinations in duplicate. Store the kit at 4°C. The shelf life of the kit is 6 months, when the kit is properly stored.
Kit Contents Amount Storage
Microtiter Plate 96-well Room temp
Urease standards
0ppm
8ppm
25ppm
50ppm
100pm
200ppm
0.2ml
0.2ml
0.2ml
0.2ml
0.2ml
0.2ml 4ºC
Urease Mix 1 vial 4ºC
Alkaline Hypochlorite Solution 20ml 4ºC
Required Materials Not Provided With the Kit
Microtiter plate reader (with 620 nm absorbance filter)
Microcentrifuge
Microcentrifuge tubes
Multichannel pipet (recommended)
Warnings and Precautions
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or different lots.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulate material under the assay plates during incubation.
Make sure you are using only distilled or deionized water since water quality is very important.
When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
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