| Trade - Want to Buy |
| Topic | Dimetridazole ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | 8566711789 |
| Mobile | |
| Fax | 8568585434 |
| Country | USA |
| Date Created | 9/27/2013 12:39:25 PM |
| Message | Product Description
REAGEN™ Dimetridazole ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of dimetridazole in meat/liver/kidney,Fish ,shrimp and urine.
The unique features of the kit are:
High recovery (70% - 95%), and cost-effective extraction methods for various samples.
A quick ELISA assay (less than 1.5 hours regardless of number of samples).
High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The Dimetridazole antibody has been coated in the plate wells. During the analysis, sample is added along with the Dimetridazole -horseradish peroxidase (HRP) conjugate. If the target is present in the sample, it will compete for the Dimetridazole antibody, thereby preventing the Dimetridazole- HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of substrate((TMB), has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Dimetridazole ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Dimetridazole -coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
Dimetridazole Standards:
Negative control
0.5ng/mL
1.5 ng/mL
3 ng/mL
6 ng/mL
12 ng/mL
100ng/mL (spiking, optional)
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL 2-8C
500x HRP-Conjugated 60 L 2-8C *
HRP Conjugate Diluent 15 mL 2-8C
TMB Substrate 12mL 2-8C
20X Wash Solution ** 28 mL 2-8C
Stop Buffer ** 14 mL 2-8C
Concentrate of Sample Extraction Buffer 30 g 2-8C
Sample Dilution Buffer 20 mL x 2 2-8C
* If you are not planning to use the kit for over 1 months, store 500 x HRP-Conjugated at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Meat/Liver/Kidney/Fish 0.5
Shrimp 0.5
Urine 2.5
Specificity
Analytes Cross-Reactivity (%)
Dimetridazole 100
Metronidazole 100
Hydroxydimetridazole 32
Ronidazole 14
Ipronidazole 11
Nicarbazin 7
Halofuginone 3
Diclazuril 4
Robenidine 2
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Rotary evaporator or nitrogen gas
oscillator
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
Acetonitrile
n-hexane
deionized water
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