| Trade - Want to Buy |
| Topic | Dairy Product Pasteurization Verification Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | USA |
| Date Created | 9/27/2013 12:36:59 PM |
| Message | Product Description
REAGEN™Dairy Product Pasteurization Verification Kit is a colorimetric assay for the confirmation of pasteurization of milk, cheese and other dairy products. REAGEN™Dairy Product Pasteurization Verification Kit uses a simple, colorimetric enzyme-based assay to measure the inactivation of bovine alkaline phosphatase in milk or cheese samples to determine pasteurization. The procedure is both sensitive and rapid: permitting confirmation of pasteurization (the procedure can detect of <0.2% raw milk contamination in pasteurized milk) in about 30 min.
The unique features of the kit are:
Rapid and simple method
Minimal sample prep
High accurate and reproducible
The kit contains positive standards to verify inactivation of the enzyme and the associated successful pasteurization
Procedure Overview
REAGEN™ Dairy Product Pasteurization Verification Kit measures inactivation of the milk alkaline phosphatase enzyme which occurs during pasteurization. After a test sample is added to the reagent mix, the sample is incubated at 37°C for 30 minutes. After adding Developer reagent to stop the reaction, the samples are compared to control tubes produced using positive standards. Successful pasteurization is indicated by a lack of pink color in the samples compared to the control
Kit Contents, Storage and Shelf Life
REAGEN™ Dairy Product Pasteurization Verification Kit contains sufficient reagents to determine the pasteurization state of 30 samples. Upon receipt of the kit, place the reaction tubes at room temperature. Store the remainder of the kit at 4°C. The shelf life is 12 months when the kit is properly stored
Kit Contents Amount Storage
Reaction tubes 60 Room temp
Reagent Mix 1.6 mL 4°C
Developer 2 X 1.6 mL 4°C
Positive Control 1.6 mL 4°C
Sample Types
The assay is specifically designed for use with whole milk, reduced fat milk, light cream and cheese.
Required Materials Not Provided With the Kit
Pipet and pipet tips
•Distilled or deionized water.
•Heating block capable of heating sample tubes to 37°C.
•Butanol (for cheese samples)
•Whatman #4 (or similar) filter paper (for cheese samples)
Warnings and Precautions
Do not use the kit past the expiration date.
Do not intermix reagents from different kits or different lots.
Try to maintain a laboratory temperature of 20°–25°C (68°–77°F).
Make sure you are using only distilled or deionized water since water quality is very important.
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