| Trade - Want to Buy |
| Topic | Total Antibiotics in Milk Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
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| Country | USA |
| Date Created | 9/27/2013 2:41:45 PM |
| Message | Product Description
REAGEN™Total Antibiotics in Milk Test Kit provides an easy and simple test method for detecting total antibiotics in milk. Compared with current commercially available kits,.
the unique features of the kit are:
A quick spore growth assay (results can be read in about 3 hours).
Detection of wide range of antibiotics in milk.
High reproducibility.
High sensitivity and low detection limit.
Procedure Overview
The method is based on the inhibition of a standard microbial growth recommended by international organizations. The kit is presented in a microplate format where each well contains medium agar spread with bacillus spores. When the plate (or a strip of wells) is incubated at 65C, these bacillus spores germinate and the bacillus grows so that acids are produced, which result in the pH value changes. The color of the pH indicator in the medium changes from purple to yellow. If milk samples contain antibiotics at higher concentration than the detection limit, the bacillus will not grow and therefore the medium color remains unchanged.
Kit Contents, Storage and Shelf Life
REAGEN™Total Antibiotics in Milk Test Kit has the capacity for 96 determinations for product Reference #: 1023-01 and 288 determinations for product Reference #: 1023-02, including negative and positive controls. Return any unused plates/strips/wells to the plastic bag provided in the original package and reseal them. The shelf life is 6 months when the kit is properly stored.
RND99026-01
Kit Contents Amount Storage
Microtiter Plate 96-well plates (8 wells x 12 strips) 2-8C
Milk Negative Control Powder 1 tube Room Temperature
Milk Positive Control Powder 1 tube Room Temperature
Milk Negative Control Diluent 1.5 mL 2-8C
Milk Positive Control Diluent** 1.5 mL -20C
Aluminum Seal 2 Sheets Room Temperature
QC Certificate 1 Na
RND99026-02
Kit Contents Amount Storage
Microtiter Plate 3x96-well plates (8wells x 12 strips) 2-8C
Milk Negative Control Powder 3 tubes RoomTemperature
Milk Positive Control Powder 3 tubes RoomTemperature
Milk Negative Control Diluent 3 tubes X 1.5 mL 2-8C
Milk Positive Control Diluent** 3 tubes X 1.5 mL -20C
Aluminum Seal 6 Sheets RoomTemperature
QC Certificate 1 Na
Upon arrival, immediately store the Milk Positive Control Diluent at -20C. See storage temperature for other components. After reconstitution of the negative and positive controls, aliquot 0.1mL into separate tubes and store in freezer at -20C.
Sensitivity
Antibiotic Detection Limit (ppb) MRL (ppb)
Penicillin G 2 4
Ampicillin 3 4
Amoxicillin 4 4
Oxacillin 20 30
Cloxacillin 30 30
Gentamycin 30 100
Tylosin 50 50
Ceftiofur 50 100
Oxytetracycline 50 100
Tetracycline 50 100
Sulfamethoxazole 200 500
Required Materials Not Provided With the Kit
65C Incubator (heat block or water bath)
100 L pipettes, tips, and tubes
Reagent Preparation
Preparation of Negative Control and Positive Control
Reconstitute the Milk Negative Control Powder with 1.5 mL of Milk Negative Control Diluent.
Reconstitute the Milk Positive Control Powder with 1.5 mL of Milk Positive Control Diluent.
Vortex tubes and mix well. Be sure the milk mixture is homogenous and no clumps are present.
After the initial reconstitution, it is recommended to aliquot the negative and positive controls into 0.1 mL aliquots and stored at -20°C freezer for future use. Failing to do so may result in inaccurate readings.
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