| Trade - Want to Buy |
| Topic | Reserpine ELISA Test Kit |
| Name | NICOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
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| Country | USA |
| Date Created | 9/27/2013 2:38:54 PM |
| Message | Product Description
REAGEN™ Reserpine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Reserpine in Fish/Shrimp/Meat(Beef/Chicken/Pork),urine and serum .The unique features of the kit are:
High recovery (75 - 105%), cost-effective extraction methods.
High sensitivity (0.25 ng/g or ppb)
High reproducibility.
A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
REAGEN™ Reserpine ELISA Test Kit is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Reserpine ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Reserpine-coated Plate 1x 96-well Plate 2-8C
Reserpine Standards:
Negative control (white cap tube)
0.25ng/mL (yellow cap tube)
0.5ng/mL (orange cap tube)
2.5 ng/mL (pink cap tube)
5ng/mL (purple cap tube)
10ng/mL (blue cap tube)
100 ng/mL (spiking, red cap tube)
1.0 mL
2-8C
2-8C
Reserpine Antibody #1 10 mL
100XHRP Conjugated Antibody #2 250L
Antibody #2 Diluent ** 20 mL
20X Wash Solution 30 mL
TMB Substrate 10 mL
Stop Buffer 15 mL
10XPBS 25ml
* If you are not planning to use the kit for over 3 months, store Reserpine Antibody #1 and 100XHRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity
Sample Type Detection Limit (ng/g or ppb)
Fish/Shrimp/Meat(Beef/Chicken/Pork) 0.2
Urine/Serum 0.4
Specificity
Analytes Cross-Reactivity (%)
Reserpine 100
Promazine <0.1
Triflupromazine <0.1
Propionylpromazine <0.1
Diphenhydramine <0.1
Maprotiline <0.1
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
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