| Trade - Want to Buy |
| Topic | Zilpaterol ELISA Test Kit |
| Name | NCOLE |
| Company | REAGEN |
| Address | 102 Commerce Dr. Unit 8, Moorestown, NJ 08057 |
| Phone | |
| Mobile | |
| Fax | |
| Country | USA |
| Date Created | 9/27/2013 1:24:43 PM |
| Message | Product Description
REAGEN™ Zilpaterol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Zilpaterol in feed, meat/liver/kidney, milk and urine.. The unique features of the kit are:
Rapid (10 - 30 minutes), and organic reagent-free extraction method for various samples with high recovery (75 - 105%)
High sensitivity (0.015 ng/g or ppb) and low detection limit (0.05 ng/g or ppb for meat/tissue, 0.25 ng/g or ppb for urine and 1 ng/g or ppb for feed)
High reproducibility
A quick ELISA assay (less than 2 hours regardless of number of samples)
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Zilpaterol ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Zilpaterol-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C
Zilpaterol Standards:
Negative control (white cap tube)
0.015 ng/mL (yellow cap tube)
0.05 ng/mL (orange cap tube)
0.1 ng/mL (pink cap tube)
0.2 ng/mL (purple cap tube)
0.5 ng/mL (blue cap tube)
10 ng/mL (spiking, optional, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
2-8C
2-8C
Zilpaterol Antibody #1 12 mL 2-8C
100X HRP-Conjugated Antibody #2 250 L
Antibody #2 Diluent ** 20 mL
20X Wash Solution ** 30 mL
Stop Buffer ** 14 mL
TMB Substrate ** 10 mL
10X PBS **
20X Sample Extraction Buffer (Optional)
Sample Balance Buffer I (Optional)
Sample Balance Buffer II (Optional)
Liver Extraction Buffer (Optional)
Beta-Agonist Clean Up Buffer I (Optional)
Beta-Agonist Clean Up Buffer II (Optional)
Beta-Agonist Clean Up Buffer III (Optional) 25 mL
10 mL
10 mL
62 g
10 mL
5 mL
5 mL
28 mL
* If you are not planning to use the kit for over 3 months, store Zilpaterol Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer.
Sensitivity (Detection Limit)
Sample Type Detection Limit (g/g or ppb)
Feed 1
Meat/Liver/Kidney 0.05
Milk 0.25
Urine 0.25
Specificity (Cross-Reactivity)
Analytes Cross-Reactivity (%)
Zilpaterol 100
Mabuterol 5.8
Mapenterol 4.2
Tolubuterol 2.8
Terbutaline 2.3
Cimbuterol 1.7
Salbutamol 1.0
Cimaterol 0.6
Pirbuterol 0.4
Isoprenaline 0.2
Required Materials Not Provided With the Kit
Microtiter plate reader (450 nm)
Incubator
Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
Rotary evaporator or nitrogen gas
Vortex mixer (e.g. Gneie Vortex mixer from VWR)
10, 20, 100 and 1000 L pipettes
Multi-channel pipette: 50-300 L (Optional)
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